Input files (input structure, topology, Plumed input) for a simple simulation used to demonstrate that free energy surface can be calculated from Flying Gaussians results using Umbrella Sampling reweighing, despite the dynamic nature of the bias potential. Tested on OpenMPI4.0.0, Gromacs 2018.5 and Plumed2.5.0. Script provided. Input files: AceAlaNme.top - Gromacs topology of Ace-Ala-Nme (alanine dipeptide) in Amber99SB force field. fg.mdp - Gromacs instruction file (10 ns, 300 K). In order to replicate the data in the article it is necessary to prolong the simulation. frame0.gro ... frame19.gro - Input structures in Gromacs format for replicas 1-20. Generated by a short MD simulation. getbias.R - R analysis file to calculate and print evolution of the bias potential (run by R --no-save << getbias.R on results of otfr.py) getfes.R - R analysis file to calculate and print evolution of the free energy surface (run by R --no-save << getfes.R on results of otfr.py) justcommands.sh - script to run the simulations (can be updated to specify software location) otfr/otfr.py - script to run Umbrella Sampling reweighing (run by ./otfr.py) plumed.dat - Plumed input file runit.sh - actual script used to run the simulation