Input files (input structures, topology, Plumed input) for simulations used to demonstrate functionality of Close Structure algorithm (J. Chem. Phys. 2017, 146, 115101). The method replaces frequent calculations of root mean square deviations in Path collective variables or Property Map collective variables. This accelerates simulations. Input files for metadynamics simulation of cyclooctane derivative in vacuum and Trp-cage in implicit solvent with two or three, respectively, Isomap CVs (with and without Close Structure). Tested on OpenMPI4.0.0, Gromacs 2018.5 and Plumed2.5.0. Scripts provided. Directories: cyclo/close - metadynamics simulation of cyclooctane derivative in vacuum with three Isomap CVs with Close Structure cyclo/normal - metadynamics simulation of cyclooctane derivative in vacuum with three Isomap CVs without Close Structure trpcage/close - metadynamics simulation of Trp-cage in implicit solvent with two Isomap CVs with Close Structure trpcage/normal - metadynamics simulation of cTrp-cage in implicit solvent with two Isomap CVs without Close Structure Files: after_em.gro - cyclooctane input structure after minimization after_em1.gro - Trp-cage input structure after minimization COLVAR - collective variable record HILLS - hills record ions.itp - ion topology justcommands.sh - commands to be run md1.mdp - Gromacs simulation command file for Trp-cage, longer simulations are needed to reproduce the results of the article molecule_gmx.top - cyclooctane topology mtd.mdp - Gromacs simulation command file for cyclooctane, longer simulations are needed to reproduce the results of the article plumed.dat - plumed input file for Trp-cage plumed_v2.dat - plumed input file for cyclooctane reference.pdb - reference coordinates with input file for cyclooctane reframe.pdb - reference coordinates with input file for Trp-cage runit.sh - actual script used in the simulation trpcage.top - Trp-cage topology