Molecular dynamics simulation trajectories of binding of N-acetylglucosamine mono- ((GlcNAc)1), di- ((GlcNAc)2) and trisaccharide ((GlcNAc)3) onto hevein domain HEV32. Systems containing one molecule of HEV32, one molecule of an oligosaccharide and 2271-2287 water molecules were simulated for 2 microseconds. Two replicates were done for each system. Binding of oligosaccharides into their experimentally verified binding sites was observed in three of six simulations. Gromacs trajectories do not contain water molecules and are are sampled every 100 ps. Gromacs structures (*.gro) and trajectories (*.xtc) can be opened in Gromacs (analysis), Visual Molecular Dynamics (visualization) or other software or libraries (e.g. Plumed, MDtraj). mono1/after_mdp3nw.gro The initial structure of the first replica of the simulation of HEV32 with (GlcNAc)1. mono1/md.xtc The trajectory of the first replica of the simulation of HEV32 with (GlcNAc)1. mono2/after_mdp3nw.gro The initial structure of the second replica of the simulation of HEV32 with (GlcNAc)1. mono2/md.xtc The trajectory of the second replica of the simulation of HEV32 with (GlcNAc)1. di1/after_mdp3nw.gro The initial structure of the first replica of the simulation of HEV32 with (GlcNAc)2. di1/md.xtc The trajectory of the first replica of the simulation of HEV32 with (GlcNAc)2. di2/after_mdp3nw.gro The initial structure of the second replica of the simulation of HEV32 with (GlcNAc)2. di2/md.xtc The trajectory of the second replica of the simulation of HEV32 with (GlcNAc)2. tri1/after_mdp3nw.gro The initial structure of the first replica of the simulation of HEV32 with (GlcNAc)3. tri1/md.xtc The trajectory of the first replica of the simulation of HEV32 with (GlcNAc)3. tri2/after_mdp3nw.gro The initial structure of the second replica of the simulation of HEV32 with (GlcNAc)3. tri2/md.xtc The trajectory of the second replica of the simulation of HEV32 with (GlcNAc)3. md5sum.log MD5 sums of files.