Property map collective variable as a useful tool for force field correction Dalibor Trapl, Martin Krupička, Vladimir Višňovský, Jana Hozzová, Jaroslav Olha, Aleš Křenek, and Vojtěch Spiwok Molecular mechanics potentials for small molecules suffer inaccuracies. To apply corrections we used a concept called property map to calculate corrections. It was calculated as a sum of [correction_i exp(-lambda D(x, x_i))] divided by the sum of [exp(-lambda D(x, x_i))], where correction_i is the difference between the accurate and inaccurate potential for i-th landmark structure x_i, lambda is a chosen prefactor, D is a distance (e.g. RMSD or MSD) and x are atomic coordinates. The concept was tested on alanine dipeptide (all combinations of 7 force fields, one used as a model of accurate and one as inaccurate). Next it was applied on an anticancer drug Imatinib (General AMBER Force Field corrected to DFT). Simulations were carried out in Gromacs 2016.4. Correction was Implemented using Plumed 2.4. DFT energies were calculated by ORCA 4.0 at the BP86/def2-TZVP level of theory. aladip/ Each directory contains Gromacs topology (topol.top), input structures (npt.cpt, npt.gro) and instructions (md.mdp), property map input and reference structure (plumed.dat, reference.pdb), gromacs_commands.sh script to run the simulations for metadynamics simulations and output HILLS file. FES_03to03_wat/ Free energy surface of alanine dipeptide in Amber03 corrected to Amber03. FES_03to94_wat/ Free energy surface of alanine dipeptide in Amber03 corrected to Amber94. FES_03to96_wat/ Free energy surface of alanine dipeptide in Amber03 corrected to Amber96. FES_03to99SBILDN_wat/ Free energy surface of alanine dipeptide in Amber03 corrected to Amber99SBILDN. FES_03to99SB_wat/ Free energy surface of alanine dipeptide in Amber03 corrected to Amber99SB. FES_03to99_wat/ Free energy surface of alanine dipeptide in Amber03 corrected to Amber99. FES_03toGS_wat/ Free energy surface of alanine dipeptide in Amber03 corrected to AmberGS. FES_94to03_wat/ Free energy surface of alanine dipeptide in Amber94 corrected to Amber03. FES_94to94_wat/ Free energy surface of alanine dipeptide in Amber94 corrected to Amber94. FES_94to96_wat/ Free energy surface of alanine dipeptide in Amber94 corrected to Amber96. FES_94to99SBILDN_wat/ Free energy surface of alanine dipeptide in Amber94 corrected to Amber99SBILDN. FES_94to99SB_wat/ Free energy surface of alanine dipeptide in Amber94 corrected to Amber99SB. FES_94to99_wat/ Free energy surface of alanine dipeptide in Amber94 corrected to Amber99. FES_94toGS_wat/ Free energy surface of alanine dipeptide in Amber94 corrected to AmberGS. FES_96to03_wat/ Free energy surface of alanine dipeptide in Amber96 corrected to Amber03. FES_96to94_wat/ Free energy surface of alanine dipeptide in Amber96 corrected to Amber94. FES_96to96_wat/ Free energy surface of alanine dipeptide in Amber96 corrected to Amber96. FES_96to99SBILDN_wat/ Free energy surface of alanine dipeptide in Amber96 corrected to Amber99SBILDN. FES_96to99SB_wat/ Free energy surface of alanine dipeptide in Amber96 corrected to Amber99SB. FES_96to99_wat/ Free energy surface of alanine dipeptide in Amber96 corrected to Amber99. FES_96toGS_wat/ Free energy surface of alanine dipeptide in Amber96 corrected to AmberGS. FES_99SBILDNto03_wat/ Free energy surface of alanine dipeptide in Amber99SBILDN corrected to Amber03. FES_99SBILDNto94_wat/ Free energy surface of alanine dipeptide in Amber99SBILDN corrected to Amber94. FES_99SBILDNto96_wat/ Free energy surface of alanine dipeptide in Amber99SBILDN corrected to Amber96. FES_99SBILDNto99SBILDN_wat/ Free energy surface of alanine dipeptide in Amber99SBILDN corrected to Amber99SBILDN. FES_99SBILDNto99SB_wat/ Free energy surface of alanine dipeptide in Amber99SBILDN corrected to Amber99SB. FES_99SBILDNto99_wat/ Free energy surface of alanine dipeptide in Amber99SBILDN corrected to Amber99. FES_99SBILDNtoGS_wat/ Free energy surface of alanine dipeptide in Amber99SBILDN corrected to AmberGS. FES_99SBto03_wat/ Free energy surface of alanine dipeptide in Amber99SB corrected to Amber03. FES_99SBto94_wat/ Free energy surface of alanine dipeptide in Amber99SB corrected to Amber94. FES_99SBto96_wat/ Free energy surface of alanine dipeptide in Amber99SB corrected to Amber96. FES_99SBto99SBILDN_wat/ Free energy surface of alanine dipeptide in Amber99SB corrected to Amber99SBILDN. FES_99SBto99SB_wat/ Free energy surface of alanine dipeptide in Amber99SB corrected to Amber99SB. FES_99SBto99_wat/ Free energy surface of alanine dipeptide in Amber99SB corrected to Amber99. FES_99SBtoGS_wat/ Free energy surface of alanine dipeptide in Amber99SB corrected to AmberGS. FES_99to03_wat/ Free energy surface of alanine dipeptide in Amber99 corrected to Amber03. FES_99to94_wat/ Free energy surface of alanine dipeptide in Amber99 corrected to Amber94. FES_99to96_wat/ Free energy surface of alanine dipeptide in Amber99 corrected to Amber96. FES_99to99SBILDN_wat/ Free energy surface of alanine dipeptide in Amber99 corrected to Amber99SBILDN. FES_99to99SB_wat/ Free energy surface of alanine dipeptide in Amber99 corrected to Amber99SB. FES_99to99_wat/ Free energy surface of alanine dipeptide in Amber99 corrected to Amber99. FES_99toGS_wat/ Free energy surface of alanine dipeptide in Amber99 corrected to AmberGS. FES_GSto03_wat/ Free energy surface of alanine dipeptide in AmberGS corrected to Amber03. FES_GSto94_wat/ Free energy surface of alanine dipeptide in AmberGS corrected to Amber94. FES_GSto96_wat/ Free energy surface of alanine dipeptide in AmberGS corrected to Amber96. FES_GSto99SBILDN_wat/ Free energy surface of alanine dipeptide in AmberGS corrected to Amber99SBILDN. FES_GSto99SB_wat/ Free energy surface of alanine dipeptide in AmberGS corrected to Amber99SB. FES_GSto99_wat/ Free energy surface of alanine dipeptide in AmberGS corrected to Amber99. FES_GStoGS_wat/ Free energy surface of alanine dipeptide in AmberGS corrected to AmberGS. fes/ Collected free energy surfaces (files with 65536 lines to be converted to 256x256 grid), regions with free energy >30 kJ/mol were set to 30 kJ/mol. dftb/ CP2K input files for metadynamics at the DFTB/MM level (MD input and energy input files, input structre, AMBER MM topology etc.) for a two-step equilibration and metadynamics. rmse.R R script to calculate RMSE between precalculated corrected free energy surfaces located in fes/ and reference free energy surfaces located in fes/control diffs.txt Output of rmse.R script, RMSE values between corrected free energy surfaces and control reference surfaces imatinib/ without_correction/ Gromacs topology (MOL_GMX.top), input structures (npt.cpt, npt.gro) and instructions (mtd.mdp) for molecular dynamics simulations of Imatinib in water. correction/ Gromacs topology (MOL_GMX.top), input structures (npt.cpt, npt.gro), instructions (mtd.mdp), property map input and reference structure (plumed.dat, reference.pdb) for molecular dynamics simulations of Imatinib in water.